Summary
Type: SPE
Application #: 305390
Catalog#: 730950 - SPE Cartridges, Chromabond, HR-XA, 3 ml, 60 mg
Detection:
Conditions: Column conditioning: 1 mL methanol, then 1 mL ammonium hydroxide solution (w(NH3) = 5 %)
Sample application: slowly force or aspirate 1 mL sample through the column
Washing: 1 mL ammonium hydroxide solution (w(NH3) = 5 %), then 1 mL methanol
Elution: 2 x 1 mL ethyl acetate with 5 % formic acid
Evaporation: nitrogen stream with slight heating to dryness
Reconstitution: HPLC solvent
Authors: SPE department
Source: pesticide metabolites; polymeric anion exchanger